Free fd2400L - Federal

DEPARTMENT OF HEALTH AND HUMAN SERVICES PUBLIC HEALTH SERVICE FOOD AND DRUG ADMINISTRATION

LABORATORY

LOCATION

LAB #

MILK LABORATORY EVALUATION FORM
DATE X = DEVIATION O = NOT USED U = UNDETERMINED NA = NOT APPLICABLE

DISINTEGRATION METHOD FOR PAPER, PAPERBOARD OR MOLDED PULP MATERIALS
[Unless otherwise stated all tolerances are ±5%]
1. Laboratory Requirements .......................................................... a. Record time and date samples received ................................. b. Record time and date samples examined ............................... 14. Transfer this 3g into a sterile disintegrator cup containing 300 mL dilution water (5g in 500 mL) ...................................... 15. Place cup on blender motor, run at high-speed 30 sec, check to insure no particles are on side of cup or trapped beneath blade ........................................................................................... 16. Continue high speed blending for a total of 2 min, depending on paper type, checking at intervals for particles on side of cup ............................................................................................... 17. Take great precautions to avoid dust, moisture, and other contaminants at all steps ..........................................................

APPARATUS AND MATERIALS
2. See Cultural Procedures, items 1-3, 10-21, 23-32 as appropriate ................................................................................. 3. Pipets, sterile ............................................................................. a. Capacity, 10 mL with 3 mm tip opening ................................. b. Or, 20 mL with large-bore tip opening ................................... 4. Pipet containers ......................................................................... a. Used for sterilization, storage, non-toxic ............................... 5. Scalpel or scissors, and forceps sterile ................................... 6. Disintegrator blender ................................................................. a. Sterile, high speed, electrically operated, corrosion-resistant cup .......................................................................................... b. Capacity, 500 mL; optionally, 1000 mL .................................. 7. 70% ethyl alcohol ...................................................................... a. In a covered container to hold scalpels, scissors and forceps 8. Dilution buffer (Cultural Procedures, item 25a,c) ................... a. In containers filled to contain 300±6 mL (or 500±10 mL) ..... 9. Sterile kraft paper or envelopes ...............................................

PLATING
18. With sterile pipet, divide 10 mL of disintegrated sample equally among 3 plates (optionally use 5 plates) .................... 19. Pour agar (see SPC, item 13), thoroughly and evenly mix with test portion in plate ............................................................

CONTROLS
20. See SPC, item 14 .......................................................................

INCUBATION
21. See SPC, item 15 ....................................................................... a. Incubate at 32±1C for 48±3 hr ...............................................

PROCEDURE
10. Not applicable when wax, plastic or metal is food contact surface ........................................................................................ 11. Use sterile cutting device, cut 100g from butt roll and transfer to sterile wrapper or envelope .................................... 12. With sterile cutting device, trim off 5 cm of the outer edge of the sample sheet ........................................................................ 13. Handling with sterile forceps, cut into 0.5 cm pieces 3g of the center portion into a sterile petri dish ................................

COUNTING COLONIES
22. See SPC, items 16, 17

REPORTS
23. Computing and Reporting Counts ............................................. a. Multiply the sum of the colonies on 3 (5) plates by 10 ......... b. Report as the number of colonies/g of stock .........................

FORM FDA 2400L (3/01)

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